› 4yis

X-ray diffraction
2.89Å resolution

Crystal Structure of LAGLIDADG Meganuclease I-CpaMI Bound to Uncleaved DNA

Released:
DOI: 10.2210/pdb4yis/pdb
PDB entry 4yis coloured by chain, front view.
PDB entry 4yis coloured by chain, side view.
PDB entry 4yis coloured by chain, top view.
Source organisms:
Primary publication:
Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Lambert AR, Hallinan JP, Shen BW, Chik JK, Bolduc JM, Kulshina N, Robins LI, Kaiser BK, Jarjour J, Havens K, Scharenberg AM, Stoddard BL
Structure 24 862-73 (2016)
PMID: 27133026

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118321 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 33.65 KDa
Source organism: Cryphonectria parasitica
Expression system: Escherichia coli
UniProt:
  • Canonical: O20960 (Residues: 122-416; Coverage: 71%)
Gene names: mtSrRNA I4, orf416
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA (28-MER) Chains: C, E
Molecule details ›
Chains: C, E
Length: 28 nucleotides
Theoretical weight: 8.44 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (28-MER) Chains: D, F
Molecule details ›
Chains: D, F
Length: 28 nucleotides
Theoretical weight: 8.75 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 75.869Å b: 91.799Å c: 139.636Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.274
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering.
Guha et al. (2017)
4 more

1 mention without citation

Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Lambert et al. (2016)