› 4yiv

X-ray diffraction
1.93Å resolution

Crystal structure of engineered TgAMA1 lacking the DII loop

Released:
DOI: 10.2210/pdb4yiv/pdb
PDB entry 4yiv coloured by chain, front view.
PDB entry 4yiv coloured by chain, side view.
PDB entry 4yiv coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
An Extended Surface Loop on Toxoplasma gondii Apical Membrane Antigen 1 (AMA1) Governs Ligand Binding Selectivity.
Parker ML, Boulanger MJ
PLoS One 10 e0126206 (2015)
PMID: 25955165

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apical membrane antigen AMA1 Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 46.06 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Trichoplusia ni
UniProt:
  • Canonical: S8GKS3 (Residues: 64-339, 360-484; Coverage: 71%)
Gene name: TGME49_255260
Sequence domains: Apical membrane antigen 1
Structure domains: Hepatocyte Growth Factor

Ligands and Environments

5 bound ligands:
Ligand NAG 1 x NAG
Ligand CD 2 x CD
Ligand CL 2 x CL
Ligand CO 3 x CO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P41212
Unit cell:
a: 89.05Å b: 89.05Å c: 124.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.195
Expression system: Trichoplusia ni

Quick links

Citations

9 citation in other articles

Dissecting the interface between apicomplexan parasite and host cell: Insights from a divergent AMA-RON2 pair.
Parker et al. (2016)
8 more

1 mention without citation

An Extended Surface Loop on Toxoplasma gondii Apical Membrane Antigen 1 (AMA1) Governs Ligand Binding Selectivity.
Parker et al. (2015)