› 4yk7

X-ray diffraction
2.6Å resolution

Crystal structure of S-hydroxynitrile lyase from Manihot esculenta (His103Leu)

Released:
DOI: 10.2210/pdb4yk7/pdb
PDB entry 4yk7 coloured by chain, front view.
PDB entry 4yk7 coloured by chain, side view.
PDB entry 4yk7 coloured by chain, top view.
Source organism: Manihot esculenta
Entry authors: Dadashipour M, Nakano S, Asano Y

Function and Biology Details

Reaction catalysed: 
An aliphatic (S)-hydroxynitrile = cyanide + an aliphatic aldehyde or ketone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-hydroxynitrile lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 258 amino acids
Theoretical weight: 29.38 KDa
Source organism: Manihot esculenta
Expression system: Escherichia coli
UniProt:
  • Canonical: P52705 (Residues: 1-258; Coverage: 100%)
Gene name: HNL
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 86.469Å b: 88.098Å c: 135.213Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.24 0.262
Expression system: Escherichia coli

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