› 4ym4

X-ray diffraction
3.12Å resolution

Truncated Human TIFA in complex with its Thr9 phosphorylated N-terminal peptide 1-15

Released:
DOI: 10.2210/pdb4ym4/pdb
PDB entry 4ym4 coloured by chain, front view.
PDB entry 4ym4 coloured by chain, side view.
PDB entry 4ym4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Uncovering the Mechanism of Forkhead-Associated Domain-Mediated TIFA Oligomerization That Plays a Central Role in Immune Responses.
Weng JH, Hsieh YC, Huang CC, Wei TY, Lim LH, Chen YH, Ho MR, Wang I, Huang KF, Chen CJ, Tsai MD
Biochemistry 54 6219-29 (2015)
PMID: 26389808

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188412 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TRAF-interacting protein with FHA domain-containing protein A Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CG3 (Residues: 10-150; Coverage: 77%)
Gene names: T2BP, TIFA
Sequence domains: FHA domain
TRAF-interacting protein with FHA domain-containing protein A Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96CG3 (Residues: 1-12; Coverage: 7%)
Gene names: T2BP, TIFA

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 5 x MSE
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P4232
Unit cell:
a: 113.205Å b: 113.205Å c: 113.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

FHA domains: Phosphopeptide binding and beyond.
Almawi et al. (2017)
2 more