› 4ymy

X-ray diffraction
1Å resolution

Crystal structure of mutant nitrobindin M75A/H76L/Q96C/M148L/H158A (NB11) from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb4ymy/pdb
PDB entry 4ymy coloured by chain, front view.
PDB entry 4ymy coloured by chain, side view.
PDB entry 4ymy coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
A Highly Active Biohybrid Catalyst for Olefin Metathesis in Water: Impact of a Hydrophobic Cavity in a beta-Barrel Protein
Sauer DF, Himiyama T, Tachikawa K, Fukumoto K, Onoda A, Mizohata E, Inoue T, Bocola M, Schwaneberg U, Hayashi T, Okuda J
ACS Catalysis 5 7519-7522 (2015)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxynitrite isomerase Rv2717c Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 19.35 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O64527 (Residues: 2-166; Coverage: 99%)
Gene names: At1g79260, YUP8H12R.14
Sequence domains: THAP4-like, heme-binding beta-barrel domain
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21212
Unit cell:
a: 59.654Å b: 79.285Å c: 36.409Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.123 0.136
Expression system: Escherichia coli BL21(DE3)

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