› 4ypi

X-ray diffraction
3.71Å resolution

Structure of Ebola virus nucleoprotein N-terminal fragment bound to a peptide derived from Ebola VP35

Released:
DOI: 10.2210/pdb4ypi/pdb
Model geometry
Fit model/data
PDB entry 4ypi coloured by chain, front view.
PDB entry 4ypi coloured by chain, side view.
PDB entry 4ypi coloured by chain, top view.
Source organism: Ebola virus - Mayinga, Zaire, 1976
Primary publication:
An Intrinsically Disordered Peptide from Ebola Virus VP35 Controls Viral RNA Synthesis by Modulating Nucleoprotein-RNA Interactions.
Leung DW, Borek D, Luthra P, Binning JM, Anantpadma M, Liu G, Harvey IB, Su Z, Endlich-Frazier A, Pan J, Shabman RS, Chiu W, Davey RA, Otwinowski Z, Basler CF, Amarasinghe GK
Cell Rep 11 376-89 (2015)
PMID: 25865894

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148341 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 348 amino acids
Theoretical weight: 38.95 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18272 (Residues: 38-385; Coverage: 47%)
Gene name: NP
Sequence domains: Ebola nucleoprotein
Polymerase cofactor VP35 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 28 amino acids
Theoretical weight: 3.09 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Not provided
UniProt:
  • Canonical: Q05127 (Residues: 20-47; Coverage: 8%)
Gene name: VP35

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: F222
Unit cell:
a: 149.752Å b: 194.818Å c: 347.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.253 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

18 review citations

How order and disorder within paramyxoviral nucleoproteins and phosphoproteins orchestrate the molecular interplay of transcription and replication.
Longhi et al. (2017)
17 more

17 mentions without citation

Virus and host interactions critical for filoviral RNA synthesis as therapeutic targets.
Basler et al. (2019)
16 more