› 4ypv

X-ray diffraction
1.85Å resolution

High-resolution structure of a metagenome-derived esterase Est8

Released:
DOI: 10.2210/pdb4ypv/pdb
PDB entry 4ypv coloured by chain, front view.
PDB entry 4ypv coloured by chain, side view.
PDB entry 4ypv coloured by chain, top view.
Source organism: Parvibaculum
Primary publication:
From a metagenomic source to a high-resolution structure of a novel alkaline esterase.
Pereira MR, Maester TC, Mercaldi GF, de Macedo Lemos EG, Hyvönen M, Balan A
Appl Microbiol Biotechnol 101 4935-4949 (2017)
PMID: 28331945

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Est8 Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 37.05 KDa
Source organism: Parvibaculum
Expression system: Escherichia coli BL21(DE3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3121
Unit cell:
a: 85.496Å b: 85.496Å c: 98.665Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.15 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Precision enzyme discovery through targeted mining of metagenomic data.
Ariaeenejad et al. (2024)
6 other articles

14 mentions without citation

Structural Basis for the Strict Substrate Selectivity of the Mycobacterial Hydrolase LipW.
McKary et al. (2016)
13 more