› 4ys0

X-ray diffraction
1.9Å resolution

Conformational changes of the clamp of the protein translocation ATPase SecA from Thermotoga maritima

Released:
DOI: 10.2210/pdb4ys0/pdb
PDB entry 4ys0 coloured by chain, front view.
PDB entry 4ys0 coloured by chain, side view.
PDB entry 4ys0 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Conformational Changes of the Clamp of the Protein Translocation ATPase SecA.
Chen Y, Bauer BW, Rapoport TA, Gumbart JC
J Mol Biol 427 2348-59 (2015)
PMID: 25982945

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecA Chain: A
Molecule details ›
Chain: A
Length: 824 amino acids
Theoretical weight: 95.17 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1R4 (Residues: 1-816; Coverage: 94%)
Gene names: TM_1578, secA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 64.404Å b: 110.16Å c: 115.863Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.226
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein export through the bacterial Sec pathway.
Tsirigotaki et al. (2017)
3 more

5 mentions without citation

The Sec System: Protein Export in Escherichia coli.
Crane et al. (2017)
4 more