› 4yuy

X-ray diffraction
1.58Å resolution

Crystal structure of Trypanosoma cruzi spermidine synthase in complex with isoquinolin-1-amine

Released:
DOI: 10.2210/pdb4yuy/pdb
Model geometry
Fit model/data
PDB entry 4yuy coloured by chain, front view.
PDB entry 4yuy coloured by chain, side view.
PDB entry 4yuy coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Primary publication:
Structural insights into the novel inhibition mechanism of Trypanosoma cruzi spermidine synthase.
Amano Y, Namatame I, Tateishi Y, Honboh K, Tanabe E, Niimi T, Sakashita H
Acta Crystallogr D Biol Crystallogr 71 1879-89 (2015)
PMID: 26327378

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PABS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.1 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DA73 (Residues: 1-296; Coverage: 100%)
Gene name: Tc00.1047053510339.50
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand S4M 2 x S4M
Ligand 1SQ 2 x 1SQ
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 44.766Å b: 94.108Å c: 68.18Å
α: 90° β: 100.41° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Polyamine-trypanothione pathway: an update.
Ilari et al. (2017)
2 more

1 mention without citation

Polyamines in protozoan pathogens.
Phillips MA. (2018)