› 4yvb

X-ray diffraction
1.35Å resolution

Structure of D128N streptavidin

Released:
DOI: 10.2210/pdb4yvb/pdb
PDB entry 4yvb coloured by chain, front view.
PDB entry 4yvb coloured by chain, side view.
PDB entry 4yvb coloured by chain, top view.
Source organism: Streptomyces avidinii
Entry authors: Baugh L, Le Trong I, Stayton PS, Stenkamp RE, Lybrand TP

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 120 amino acids
Theoretical weight: 12.69 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 40-159; Coverage: 76%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand BTN 4 x BTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 50.597Å b: 98.95Å c: 52.638Å
α: 90° β: 112.58° γ: 90°
R-values:
R R work R free
0.129 0.127 0.17
Expression system: Escherichia coli

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