› 4ywu

X-ray diffraction
2.4Å resolution

Structural insight into the substrate inhibition mechanism of NADP+-dependent succinic semialdehyde dehydrogenase from Streptococcus pyogenes

Released:
DOI: 10.2210/pdb4ywu/pdb
PDB entry 4ywu coloured by chain, front view.
PDB entry 4ywu coloured by chain, side view.
PDB entry 4ywu coloured by chain, top view.
Source organism: Streptococcus pyogenes MGAS1882
Primary publication:
Structural insight into the substrate inhibition mechanism of NADP(+)-dependent succinic semialdehyde dehydrogenase from Streptococcus pyogenes.
Jang EH, Park SA, Chi YM, Lee KS
Biochem Biophys Res Commun 461 487-93 (2015)
PMID: 25888791

Function and Biology Details

Reaction catalysed: 
Succinate semialdehyde + NADP(+) + H(2)O = succinate + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 465 amino acids
Theoretical weight: 51.86 KDa
Source organism: Streptococcus pyogenes MGAS1882
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0J9X1M8 (Residues: 1-465; Coverage: 100%)
Gene name: gabD
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SSN 2 x SSN
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P43212
Unit cell:
a: 134.965Å b: 134.965Å c: 172.551Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Substrate inhibition by the blockage of product release and its control by tunnel engineering.
Kokkonen et al. (2021)
3 more