› 4yx2

X-ray diffraction
2.19Å resolution

Crystal structure of Bovine prion protein complexed with POM1 FAB

Released:
DOI: 10.2210/pdb4yx2/pdb
Model geometry
Fit model/data
PDB entry 4yx2 coloured by chain, front view.
PDB entry 4yx2 coloured by chain, side view.
PDB entry 4yx2 coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structural and molecular dynamical studies of the globular domains of cow, deer, elk and Syrian hamster prion proteins.
Baral PK, Swayampakula M, Aguzzi A, James MN
J Struct Biol 192 37-47 (2015)
PMID: 26320075

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230430 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Major prion protein Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.49 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10279 (Residues: 103-242; Coverage: 58%)
Gene names: PRNP, PRP
Sequence domains: Prion/Doppel alpha-helical domain
POM1 FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
POM1 FAB LIGHT CHAIN Chain: L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2
Unit cell:
a: 89.674Å b: 99.764Å c: 75.942Å
α: 90° β: 93.08° γ: 90°
R-values:
R R work R free
0.218 0.216 0.263
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

3 review citations

Conformational selection in amyloid-based immunotherapy: Survey of crystal structures of antibody-amyloid complexes.
Ma et al. (2016)
2 more