4yxb

X-ray diffraction
2.56Å resolution

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-150743 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Flagellar motor switch protein FliM; Flagellar motor switch protein FliN Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P26418 (Residues: 245-334; Coverage: 27%)
  • Canonical: P26419 (Residues: 5-137; Coverage: 97%)
Gene names: STM1976, STM1977, cheC2, fla AII, fla QII, flaN, fliM, fliN, motD
Sequence domains:
Flagellar motor switch protein FliN Chain: C
Molecule details ›
Chain: C
Length: 6 amino acids
Theoretical weight: 691 Da
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P26419 (Residues: 38-43; Coverage: 4%)
Gene names: STM1977, flaN, fliN, motD
Flagellar motor switch protein FliN Chain: D
Molecule details ›
Chain: D
Length: 80 amino acids
Theoretical weight: 8.91 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. 798
Expression system: Escherichia coli
UniProt:
  • Canonical: P26419 (Residues: 67-137; Coverage: 52%)
Gene names: STM1977, flaN, fliN, motD
Sequence domains: Type III flagellar switch regulator (C-ring) FliN C-term
Ambiguous peptide density Chain: E
Molecule details ›
Chain: E
Length: 6 amino acids
Theoretical weight: 529 Da
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 75.15Å b: 81.5Å c: 89.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.218 0.259
Expression system: Escherichia coli