› 4yxc

X-ray diffraction
2.3Å resolution

Complex of FliM(SPOA)::FliN fusion protein and FliH(APAR)::T4lysozyme fusion protein

Released:
DOI: 10.2210/pdb4yxc/pdb
PDB entry 4yxc coloured by chain, front view.
PDB entry 4yxc coloured by chain, side view.
PDB entry 4yxc coloured by chain, top view.
Source organisms:
Primary publication:
A common assembly module in injectisome and flagellar type III secretion sorting platforms.
Notti RQ, Bhattacharya S, Lilic M, Stebbins CE
Nat Commun 6 7125 (2015)
PMID: 25994170

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133023 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.8 KDa
Source organism: Salmonella enterica subsp. enterica serovar Enteritidis str. SE30663
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme
Flagellar motor switch protein FliM; Flagellar motor switch protein FliN Chain: B
Molecule details ›
Chain: B
Length: 227 amino acids
Theoretical weight: 24.9 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P26418 (Residues: 245-334; Coverage: 27%)
  • Canonical: P26419 (Residues: 5-137; Coverage: 97%)
Gene names: STM1976, STM1977, cheC2, fla AII, fla QII, flaN, fliM, fliN, motD
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 43.21Å b: 76.37Å c: 119.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.197 0.262
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Assembly, structure, function and regulation of type III secretion systems.
Deng et al. (2017)
12 more

4 mentions without citation

The Architectural Dynamics of the Bacterial Flagellar Motor Switch.
Khan S. (2020)
3 more