› 4yxj

X-ray diffraction
2.55Å resolution

Structure of Thermotoga maritima DisA in complex with ApCpp

Released:
DOI: 10.2210/pdb4yxj/pdb
PDB entry 4yxj coloured by chain, front view.
PDB entry 4yxj coloured by chain, side view.
PDB entry 4yxj coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structural analysis of the diadenylate cyclase reaction of DNA-integrity scanning protein A (DisA) and its inhibition by 3'-dATP.
Müller M, Deimling T, Hopfner KP, Witte G
Biochem J 469 367-74 (2015)
PMID: 26014055

Function and Biology Details

Reaction catalysed: 
2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA integrity scanning protein DisA Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 42.76 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WY43 (Residues: 1-357; Coverage: 100%)
Gene names: TM_0200, disA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand APC 2 x APC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P4212
Unit cell:
a: 108.25Å b: 108.25Å c: 166.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.243
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Cyclic dinucleotide (c-di-GMP, c-di-AMP, and cGAMP) signalings have come of age to be inhibited by small molecules.
Opoku-Temeng et al. (2016)
1 more

2 mentions without citation

Making and Breaking of an Essential Poison: the Cyclases and Phosphodiesterases That Produce and Degrade the Essential Second Messenger Cyclic di-AMP in Bacteria.
Commichau et al. (2019)
1 more