› 4yyo

X-ray diffraction
1.77Å resolution

Resting state of rat cysteine dioxygenase C164S variant

Released:
DOI: 10.2210/pdb4yyo/pdb
PDB entry 4yyo coloured by chain, front view.
PDB entry 4yyo coloured by chain, side view.
PDB entry 4yyo coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Influence of cysteine 164 on active site structure in rat cysteine dioxygenase.
Fellner M, Siakkou E, Faponle AS, Tchesnokov EP, de Visser SP, Wilbanks SM, Jameson GN
J Biol Inorg Chem 21 501-10 (2016)
PMID: 27193596

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.04 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 57.8Å b: 57.8Å c: 123.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Mechanistic Insight on the Activity and Substrate Selectivity of Nonheme Iron Dioxygenases.
de Visser SP. (2018)
10 other articles