› 4yzh

X-ray diffraction
2Å resolution

Structure of the Arabidopsis TAP38/PPH1 in complex with pLhcb1 phosphopeptide substrate

Released:
DOI: 10.2210/pdb4yzh/pdb
PDB entry 4yzh coloured by chain, front view.
PDB entry 4yzh coloured by chain, side view.
PDB entry 4yzh coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural Mechanism Underlying the Specific Recognition between the Arabidopsis State-Transition Phosphatase TAP38/PPH1 and Phosphorylated Light-Harvesting Complex Protein Lhcb1.
Wei X, Guo J, Li M, Liu Z
Plant Cell 27 1113-27 (2015)
PMID: 25888588

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143512 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein phosphatase 2C 57 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.72 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49599 (Residues: 59-351; Coverage: 76%)
Gene names: At4g27800, PP2C57, PPH1, T27E11.40, TAP38
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain
Chlorophyll a-b binding protein 2, chloroplastic Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.68 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: P0CJ48 (Residues: 36-50; Coverage: 6%)
Gene names: AB165, At1g29920, CAB2, F1N18.4, LHCB1.1, LHCP-B

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Unit cell:
a: 60.291Å b: 64.079Å c: 100.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.217
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Composition, phosphorylation and dynamic organization of photosynthetic protein complexes in plant thylakoid membrane.
Rantala et al. (2020)
4 more