› 4yzi

X-ray diffraction
2.5Å resolution

Crystal structure of blue-shifted channelrhodopsin mutant (T198G/G202A)

Released:
DOI: 10.2210/pdb4yzi/pdb
PDB entry 4yzi coloured by chain, front view.
PDB entry 4yzi coloured by chain, side view.
PDB entry 4yzi coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Atomistic design of microbial opsin-based blue-shifted optogenetics tools.
Kato HE, Kamiya M, Sugo S, Ito J, Taniguchi R, Orito A, Hirata K, Inutsuka A, Yamanaka A, Maturana AD, Ishitani R, Sudo Y, Hayashi S, Nureki O
Nat Commun 6 7177 (2015)
PMID: 25975962

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensory opsin A; Sensory opsin B Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 34.56 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8L435 (Residues: 39-245; Coverage: 30%)
  • Canonical: Q8RUT8 (Residues: 207-303; Coverage: 13%)
Gene names: CHLRE_02g085257v5, CSOA, CSOB, acop2, cop4
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand OLA 5 x OLA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2221
Unit cell:
a: 59.64Å b: 142.42Å c: 92.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.241
Expression system: Spodoptera frugiperda

Quick links

Citations

13 review citations

Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.
Govorunova et al. (2017)
12 more

5 mentions without citation

Atomistic design of microbial opsin-based blue-shifted optogenetics tools.
Kato et al. (2015)
4 more