› 4z07

X-ray diffraction
2.5Å resolution

Co-crystal structure of the tandem CNB (CNB-A/B) domains of human PKG I beta with cGMP

Released:
DOI: 10.2210/pdb4z07/pdb
PDB entry 4z07 coloured by chain, front view.
PDB entry 4z07 coloured by chain, side view.
PDB entry 4z07 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of PKG I:cGMP Complex Reveals a cGMP-Mediated Dimeric Interface that Facilitates cGMP-Induced Activation.
Kim JJ, Lorenz R, Arold ST, Reger AS, Sankaran B, Casteel DE, Herberg FW, Kim C
Structure 24 710-720 (2016)
PMID: 27066748

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-171845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 262 amino acids
Theoretical weight: 29.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13976 (Residues: 84-336; Coverage: 38%)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand PCG 5 x PCG
Ligand SO4 1 x SO4
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C222
Unit cell:
a: 73.239Å b: 202.978Å c: 134.551Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Chemosensory signal transduction in Caenorhabditis elegans.
Ferkey et al. (2021)
1 more

7 mentions without citation

Allostery, and how to define and measure signal transduction.
Nussinov et al. (2022)
6 more