› 4z0c

X-ray diffraction
2.3Å resolution

Crystal structure of TLR13-ssRNA13 complex

Released:
DOI: 10.2210/pdb4z0c/pdb
PDB entry 4z0c coloured by chain, front view.
PDB entry 4z0c coloured by chain, side view.
PDB entry 4z0c coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13.
Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D, Wang J, Wang HW, Chai J
Nat Struct Mol Biol 22 782-7 (2015)
PMID: 26323037

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180287 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Toll-like receptor 13 Chains: A, D
Molecule details ›
Chains: A, D
Length: 709 amino acids
Theoretical weight: 81.34 KDa
Source organism: Mus musculus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q6R5N8 (Residues: 69-777; Coverage: 77%)
Gene name: Tlr13
Sequence domains: Leucine rich repeat
DNA (5'-R(P*AP*CP*GP*GP*AP*AP*AP*GP*AP*CP*CP*CP*C)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 13 nucleotides
Theoretical weight: 4.16 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 20 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 112.549Å b: 115.209Å c: 167.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.244
Expression systems:
  • Insect cell expression vector pTIE1
  • Not provided

Quick links

Citations

15 review citations

Distinct and Orchestrated Functions of RNA Sensors in Innate Immunity.
Liu et al. (2020)
14 more

6 mentions without citation

Structure of fish Toll-like receptors (TLR) and NOD-like receptors (NLR).
Sahoo BR. (2020)
5 more