› 4z0n

X-ray diffraction
1.26Å resolution

Crystal Structure of a Periplasmic Solute binding protein (IPR025997) from Streptobacillus moniliformis DSM-12112 (Smon_0317, TARGET EFI-511281) with bound D-Galactose

Released:
DOI: 10.2210/pdb4z0n/pdb
PDB entry 4z0n coloured by chain, front view.
PDB entry 4z0n coloured by chain, side view.
PDB entry 4z0n coloured by chain, top view.
Source organism: Streptobacillus moniliformis DSM 12112
Entry authors: Yadava U, Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Koss J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactose/methyl-galactoside binding periplasmic protein MglB Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 37.52 KDa
Source organism: Streptobacillus moniliformis DSM 12112
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1AWX5 (Residues: 19-332; Coverage: 100%)
Gene name: Smon_0317
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

6 bound ligands:
Ligand SO4 4 x SO4
Ligand GAL 1 x GAL
Ligand ACT 1 x ACT
Ligand CA 1 x CA
Ligand EDO 1 x EDO
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 116.396Å b: 44.011Å c: 62.037Å
α: 90° β: 104.32° γ: 90°
R-values:
R R work R free
0.14 0.139 0.165
Expression system: Escherichia coli BL21(DE3)

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