› 4z0x

X-ray diffraction
2Å resolution

Structure of Hepatitis C Virus Envelope glycoprotein E2 antigenic region 434-446 bound to the broadly neutralizing antibody HC26AM

Released:
DOI: 10.2210/pdb4z0x/pdb
PDB entry 4z0x coloured by chain, front view.
PDB entry 4z0x coloured by chain, side view.
PDB entry 4z0x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Affinity maturation of a broadly neutralizing human monoclonal antibody that prevents acute hepatitis C virus infection in mice.
Keck ZY, Wang Y, Lau P, Lund G, Rangarajan S, Fauvelle C, Liao GC, Holtsberg FW, Warfield KL, Aman MJ, Pierce BG, Fuerst TR, Bailey JR, Baumert TF, Mariuzza RA, Kneteman NM, Foung SK
Hepatology 64 1922-1933 (2016)
PMID: 27641232

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233295 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody HC26AM light chain variable domain Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Antibody HC26AM heavy chain variable domain Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 13.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Envelope glycoprotein E2 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.42 KDa
UniProt:
  • Canonical: P27958 (Residues: 435-446; Coverage: 0%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 50.72Å b: 51.07Å c: 69.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.19 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Approaches, Progress, and Challenges to Hepatitis C Vaccine Development.
Bailey et al. (2019)
20 more

8 mentions without citation

Computational Modeling of Hepatitis C Virus Envelope Glycoprotein Structure and Recognition.
Guest et al. (2018)
7 more