› 4z2t

X-ray diffraction
2.45Å resolution

Crystal Structure of 2-hydroxybiphenyl 3-monooxygenase W225Y from Pseudomonas azelaica

Released:
DOI: 10.2210/pdb4z2t/pdb
PDB entry 4z2t coloured by chain, front view.
PDB entry 4z2t coloured by chain, side view.
PDB entry 4z2t coloured by chain, top view.
Source organism: Pseudomonas nitroreducens HBP1
Primary publication:
A crystal structure of 2-hydroxybiphenyl 3-monooxygenase with bound substrate provides insights into the enzymatic mechanism.
Kanteev M, Bregman-Cohen A, Deri B, Shahar A, Adir N, Fishman A
Biochim Biophys Acta 1854 1906-1913 (2015)
PMID: 26275805

Function and Biology Details

Reaction catalysed: 
2-hydroxybiphenyl + NADH + O(2) = 2,3-dihydroxybiphenyl + NAD(+) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 592 amino acids
Theoretical weight: 64.64 KDa
Source organism: Pseudomonas nitroreducens HBP1
Expression system: Escherichia coli
UniProt:
  • Canonical: O06647 (Residues: 2-586; Coverage: 100%)
Gene name: hbpA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 154.38Å b: 130.58Å c: 78.75Å
α: 90° β: 98.79° γ: 90°
R-values:
R R work R free
0.219 0.218 0.236
Expression system: Escherichia coli

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Citations

1 review citation

Monooxygenation of aromatic compounds by flavin-dependent monooxygenases.
Chenprakhon et al. (2019)
9 other articles