› 4z30

X-ray diffraction
2.71Å resolution

Crystal structure of the ROQ domain of human Roquin-2

Released:
DOI: 10.2210/pdb4z30/pdb
PDB entry 4z30 coloured by chain, front view.
PDB entry 4z30 coloured by chain, side view.
PDB entry 4z30 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Zhang Q, Fan L, Hou F, Dong A, Wang YX, Tong Y
Sci Rep 5 15660 (2015)
PMID: 26489670

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Roquin-2 Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 36.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HBD1 (Residues: 87-404; Coverage: 27%)
Gene names: MNAB, RC3H2, RNF164
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand UNX 4 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P43212
Unit cell:
a: 144.764Å b: 144.764Å c: 56.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.253
Expression system: Escherichia coli

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Citations

7 review citations

Ubiquitin signaling in immune responses.
Hu et al. (2016)
6 more

1 mention without citation

New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Zhang et al. (2015)