› 4z3k

X-ray diffraction
2.35Å resolution

Human sepiapterin reductase in complex with the cofactor NADP+ and the trypthophan metabolite xanthurenic acid

Released:
DOI: 10.2210/pdb4z3k/pdb
PDB entry 4z3k coloured by chain, front view.
PDB entry 4z3k coloured by chain, side view.
PDB entry 4z3k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid.
Haruki H, Hovius R, Pedersen MG, Johnsson K
J Biol Chem 291 652-7 (2016)
PMID: 26565027

Function and Biology Details

Reaction catalysed: 
L-erythro-7,8-dihydrobiopterin + NADP(+) = sepiapterin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sepiapterin reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 275 amino acids
Theoretical weight: 29.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35270 (Residues: 1-261; Coverage: 100%)
Gene name: SPR
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
3 bound ligands:
Ligand 4KL 4 x 4KL
Ligand SO4 6 x SO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P61
Unit cell:
a: 143.964Å b: 143.964Å c: 180.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.181 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Inflammation Effects on Motivation and Motor Activity: Role of Dopamine.
Felger et al. (2017)
12 more

2 mentions without citation

Sepiapterin reductase: Characteristics and role in diseases.
Wu et al. (2020)
1 more