› 4z3n

X-ray diffraction
2.7Å resolution

Crystal structure of the MATE transporter ClbM

Released:
DOI: 10.2210/pdb4z3n/pdb
PDB entry 4z3n coloured by chain, front view.
PDB entry 4z3n coloured by chain, side view.
PDB entry 4z3n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
MATE transport of the E. coli-derived genotoxin colibactin.
Mousa JJ, Yang Y, Tomkovich S, Shima A, Newsome RC, Tripathi P, Oswald E, Bruner SD, Jobin C
Nat Microbiol 1 15009 (2016)
PMID: 27571755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug-efflux transporter Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 55.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0P7K3 (Residues: 1-479; Coverage: 100%)
Gene names: C1Q91_005014, D3C88_26930, EPS76_05790, EWK56_23780, FPI65_12345, IFB95_001928, NCTC9075_02744, clbM, mepA_2
Sequence domains: MatE

Ligands and Environments

2 bound ligands:
Ligand OLC 7 x OLC
Ligand CAC 1 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3121
Unit cell:
a: 79.46Å b: 79.46Å c: 174.921Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.206 0.255
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
17 more

7 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
6 more