› 4z5d

X-ray diffraction
2.15Å resolution

HipB-O4 21mer complex

Released:
DOI: 10.2210/pdb4z5d/pdb
PDB entry 4z5d coloured by chain, front view.
PDB entry 4z5d coloured by chain, side view.
PDB entry 4z5d coloured by chain, top view.
Source organisms:
Entry authors: Min J, Brennan RG, Schumacher MA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118359 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Antitoxin HipB Chains: A, B
Molecule details ›
Chains: A, B
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23873 (Residues: 4-74; Coverage: 81%)
Gene names: JW1501, b1508, hipB
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*TP*TP*TP*AP*TP*CP*CP*GP*CP*GP*AP*TP*CP*GP*CP*GP*GP*AP*TP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*AP*TP*CP*CP*GP*CP*GP*AP*TP*CP*GP*CP*GP*GP*AP*TP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P3121
Unit cell:
a: 108.604Å b: 108.604Å c: 59.189Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.179 0.183
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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