› 4z7k

X-ray diffraction
3Å resolution

Crystal structure of CRISPR RNA processing endoribonuclease Cas6b

Released:
DOI: 10.2210/pdb4z7k/pdb
PDB entry 4z7k coloured by chain, front view.
PDB entry 4z7k coloured by chain, side view.
PDB entry 4z7k coloured by chain, top view.
Source organisms:
Primary publication:
A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.
Shao Y, Richter H, Sun S, Sharma K, Urlaub H, Randau L, Li H
Structure 24 547-554 (2016)
PMID: 26996962

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107283 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
DNA repair protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.56 KDa
Source organism: Methanococcus maripaludis C5
Expression system: Escherichia coli
UniProt:
  • Canonical: A4FXZ3 (Residues: 1-218; Coverage: 100%)
Gene name: MmarC5_0767
Sequence domains:
RNA/DNA Hybrid (31-MER) Chain: C
Molecule details ›
Chain: C
Length: 31 nucleotides
Theoretical weight: 9.95 KDa
Source organism: Methanococcus maripaludis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P31
Unit cell:
a: 92.122Å b: 92.122Å c: 96.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.224 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales.
Zink et al. (2020)
1 more

5 mentions without citation

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering.
Zheng et al. (2020)
4 more