› 4z7l

X-ray diffraction
3.5Å resolution

Crystal structure of Cas6b

Released:
DOI: 10.2210/pdb4z7l/pdb
PDB entry 4z7l coloured by chain, front view.
PDB entry 4z7l coloured by chain, side view.
PDB entry 4z7l coloured by chain, top view.
Source organisms:
Primary publication:
A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.
Shao Y, Richter H, Sun S, Sharma K, Urlaub H, Randau L, Li H
Structure 24 547-554 (2016)
PMID: 26996962

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107284 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
DNA repair protein Chains: A, B, D, E, G, H
Molecule details ›
Chains: A, B, D, E, G, H
Length: 218 amino acids
Theoretical weight: 25.56 KDa
Source organism: Methanococcus maripaludis C5
Expression system: Escherichia coli
UniProt:
  • Canonical: A4FXZ3 (Residues: 1-218; Coverage: 100%)
Gene name: MmarC5_0767
Sequence domains:
RNA (5'-R(*GP*CP*AP*AP*AP*AP*UP*AP*AP*CP*AP*AP*GP*C)-3') Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 14 nucleotides
Theoretical weight: 4.5 KDa
Source organism: Methanococcus maripaludis
Expression system: unidentified

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P64
Unit cell:
a: 180.57Å b: 180.57Å c: 119.022Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.243 0.29
Expression systems:
  • Escherichia coli
  • unidentified

Quick links

Citations

3 review citations

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering.
Zheng et al. (2020)
2 more

2 mentions without citation

A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.
Shao et al. (2016)
1 more