› 4z8b

X-ray diffraction
1.95Å resolution

crystal structure of a DGL mutant - H51G H131N

Released:
DOI: 10.2210/pdb4z8b/pdb
PDB entry 4z8b coloured by chain, front view.
PDB entry 4z8b coloured by chain, side view.
PDB entry 4z8b coloured by chain, top view.
Source organism: Macropsychanthus grandiflorus
Primary publication:
Quaternary structure of Dioclea grandiflora lectin assessed by equilibrium sedimentation and crystallographic analysis of recombinant mutants.
Zamora-Caballero S, Pérez A, Sanz L, Bravo J, Calvete JJ
FEBS Lett 589 2290-6 (2015)
PMID: 26226421

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lectin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 25.53 KDa
Source organism: Macropsychanthus grandiflorus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08902 (Residues: 1-237; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

5 bound ligands:
Ligand CA 1 x CA
Ligand MN 1 x MN
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
Ligand XMM 1 x XMM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: I222
Unit cell:
a: 62.35Å b: 67.08Å c: 108.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

ConA-Like Lectins: High Similarity Proteins as Models to Study Structure/Biological Activities Relationships.
Cavada et al. (2018)
1 more