› 4z9a

X-ray diffraction
2.1Å resolution

Crystal structure of Low Molecular Weight Protein Tyrosine Phosphatase isoform A complexed with phenylmethanesulfonic acid

Released:
DOI: 10.2210/pdb4z9a/pdb
PDB entry 4z9a coloured by chain, front view.
PDB entry 4z9a coloured by chain, side view.
PDB entry 4z9a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the apo form and a complex of human LMW-PTP with a phosphonic acid provide new evidence of a secondary site potentially related to the anchorage of natural substrates.
Fonseca EMB, Trivella DBB, Scorsato V, Dias MP, Bazzo NL, Mandapati KR, de Oliveira FL, Ferreira-Halder CV, Pilli RA, Miranda PCML, Aparicio R
Bioorg Med Chem 23 4462-4471 (2015)
PMID: 26117648

Function and Biology Details

Reactions catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low molecular weight phosphotyrosine protein phosphatase Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24666 (Residues: 1-158; Coverage: 100%)
Gene name: ACP1
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
Ligand PMS 1 x PMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 32.75Å b: 55.23Å c: 97.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.179 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Phosphonic acid: preparation and applications.
Sevrain et al. (2017)
1 more

1 mention without citation

Structural and Biochemical Characterization of the Two Drosophila Low Molecular Weight-Protein Tyrosine Phosphatases DARP and Primo-1.
Lee et al. (2020)