› 4zcm

X-ray diffraction
3.31Å resolution

Crystal Structure of Escherichia coli GTPase BipA/TypA Complexed with ppGpp

Released:
DOI: 10.2210/pdb4zcm/pdb
Model geometry
Fit model/data
PDB entry 4zcm coloured by chain, front view.
PDB entry 4zcm coloured by chain, side view.
PDB entry 4zcm coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and Functional Analysis of BipA, a Regulator of Virulence in Enteropathogenic Escherichia coli.
Fan H, Hahm J, Diggs S, Perry JJP, Blaha G
OpenAccess logo J Biol Chem 290 20856-20864 (2015)
PMID: 26163516

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit assembly factor BipA Chains: A, B
Molecule details ›
Chains: A, B
Length: 641 amino acids
Theoretical weight: 70.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3B2 (Residues: 1-607; Coverage: 100%)
Gene names: E2348C_4177, bipA, o591, typA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NCO 4 x NCO
Ligand G4P 2 x G4P
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 56.821Å b: 160.125Å c: 89.718Å
α: 90° β: 98.04° γ: 90°
R-values:
R R work R free
0.235 0.233 0.291
Expression system: Escherichia coli

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Citations

5 review citations

The magic dance of the alarmones (p)ppGpp.
Steinchen et al. (2016)
4 more

3 mentions without citation

Emerging and divergent roles of pyrophosphorylated nucleotides in bacterial physiology and pathogenesis.
Chau et al. (2021)
2 more