› 4zf4

X-ray diffraction
1.82Å resolution

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(Cl1Y), H148D; circular permutant (50-51)

Released:
DOI: 10.2210/pdb4zf4/pdb
PDB entry 4zf4 coloured by chain, front view.
PDB entry 4zf4 coloured by chain, side view.
PDB entry 4zf4 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP).
Oltrogge LM, Boxer SG
ACS Cent Sci 1 148-56 (2015)
PMID: 27162964

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.23 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 51-237, 4-50; Coverage: 98%)
Gene name: GFP
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 4NT 2 x 4NT

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 51.84Å b: 68.59Å c: 60.88Å
α: 90° β: 100.5° γ: 90°
R-values:
R R work R free
0.201 0.198 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Low barrier hydrogen bonds in protein structure and function.
Kemp et al. (2021)
3 more

2 mentions without citation

Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP).
Oltrogge et al. (2015)
1 more