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X-ray diffraction
2.01Å resolution

Phototoxic Fluorescent Protein KillerOrange

Released:
DOI: 10.2210/pdb4zfs/pdb
PDB entry 4zfs coloured by chain, front view.
PDB entry 4zfs coloured by chain, side view.
PDB entry 4zfs coloured by chain, top view.
Source organism: Hydrozoa
Primary publication:
Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.
Pletneva NV, Pletnev VZ, Sarkisyan KS, Gorbachev DA, Egorov ES, Mishin AS, Lukyanov KA, Dauter Z, Pletnev S
PLoS One 10 e0145740 (2015)
PMID: 26699366

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KillerRed Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 246 amino acids
Theoretical weight: 27.63 KDa
Source organism: Hydrozoa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TCH5 (Residues: 2-237; Coverage: 99%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 128.93Å b: 202.064Å c: 116.679Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.236
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Light-induced oxidant production by fluorescent proteins.
Trewin et al. (2018)
5 more

1 mention without citation

Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.
Pletneva et al. (2015)