› 4zfv

X-ray diffraction
1.5Å resolution

Lipomyces starkeyi levoglucosan kinase bound to ADP and magnesium.

Released:
DOI: 10.2210/pdb4zfv/pdb
PDB entry 4zfv coloured by chain, front view.
PDB entry 4zfv coloured by chain, side view.
PDB entry 4zfv coloured by chain, top view.
Source organism: Lipomyces starkeyi
Primary publication:
Producing glucose 6-phosphate from cellulosic biomass: structural insights into levoglucosan bioconversion.
Bacik JP, Klesmith JR, Whitehead TA, Jarboe LR, Unkefer CJ, Mark BL, Michalczyk R
J Biol Chem 290 26638-48 (2015)
PMID: 26354439

Function and Biology Details

Reaction catalysed: 
ATP + levoglucosan + H(2)O = ADP + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Levoglucosan kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 447 amino acids
Theoretical weight: 49.4 KDa
Source organism: Lipomyces starkeyi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3VI55 (Residues: 1-439; Coverage: 100%)
Sequence domains: Anhydro-N-acetylmuramic acid kinase

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 4 x MG
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 114.399Å b: 114.399Å c: 232.499Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.174
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Bioconversion of anhydrosugars: Emerging concepts and strategies.
Bacik et al. (2016)
7 other articles

1 mention without citation

Producing glucose 6-phosphate from cellulosic biomass: structural insights into levoglucosan bioconversion.
Bacik et al. (2015)