› 4zgs

X-ray diffraction
2.46Å resolution

Identification of the pyruvate reductase of Chlamydomonas reinhardtii

Released:
DOI: 10.2210/pdb4zgs/pdb
PDB entry 4zgs coloured by chain, front view.
PDB entry 4zgs coloured by chain, side view.
PDB entry 4zgs coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts.
Burgess SJ, Taha H, Yeoman JA, Iamshanova O, Chan KX, Boehm M, Behrends V, Bundy JG, Bialek W, Murray JW, Nixon PJ
Plant Cell Physiol 57 82-94 (2016)
PMID: 26574578

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative D-lactate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 386 amino acids
Theoretical weight: 42.08 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli KRX
UniProt:
  • Canonical: B0LUZ5 (Residues: 45-421; Coverage: 90%)
Gene name: CHLRE_07g324550v5
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NAD 8 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 83.76Å b: 110.92Å c: 193.16Å
α: 90° β: 90.11° γ: 90°
R-values:
R R work R free
0.205 0.202 0.254
Expression system: Escherichia coli KRX

Quick links

Citations

9 citation in other articles

Multiomics resolution of molecular events during a day in the life of Chlamydomonas.
Strenkert et al. (2019)
8 more

2 mentions without citation

Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts.
Burgess et al. (2016)
1 more