› 4zhy

X-ray diffraction
1.97Å resolution

Crystal structure of a bacterial signalling complex

Released:
DOI: 10.2210/pdb4zhy/pdb
PDB entry 4zhy coloured by chain, front view.
PDB entry 4zhy coloured by chain, side view.
PDB entry 4zhy coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1.
Li S, Li T, Xu Y, Zhang Q, Zhang W, Che S, Liu R, Wang Y, Bartlam M
Sci Rep 5 16915 (2015)
PMID: 26593397

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191376 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Negative regulator YfiR Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 20.69 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I4L4 (Residues: 1-190; Coverage: 100%)
Gene names: PA1121, yfiR
Sequence domains: YfiR/HmsC-like
Outer-membrane lipoprotein YfiB Chain: B
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 18.34 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I4L6 (Residues: 1-168; Coverage: 100%)
Gene names: PA1119, yfiB
Sequence domains: OmpA family
Structure domains: OmpA-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 118.539Å b: 160.989Å c: 43.636Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.219
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Biofilms and Cyclic di-GMP (c-di-GMP) Signaling: Lessons from Pseudomonas aeruginosa and Other Bacteria.
Valentini et al. (2016)
5 other articles

1 mention without citation

Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1.
Li et al. (2015)