› 4zil

X-ray diffraction
1.51Å resolution

Crystal structure of Rv2466c and oxidoreductase from Mycobacterium tuberculosis in its reduced state

Released:
DOI: 10.2210/pdb4zil/pdb
PDB entry 4zil coloured by chain, front view.
PDB entry 4zil coloured by chain, side view.
PDB entry 4zil coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The Redox State Regulates the Conformation of Rv2466c to Activate the Antitubercular Prodrug TP053.
Albesa-Jové D, Comino N, Tersa M, Mohorko E, Urresti S, Dainese E, Chiarelli LR, Pasca MR, Manganelli R, Makarov V, Riccardi G, Svergun DI, Glockshuber R, Guerin ME
J Biol Chem 290 31077-89 (2015)
PMID: 26546681

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin-like reductase Rv2466c Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 24.59 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53193 (Residues: 1-207; Coverage: 100%)
Gene names: P425_02568, RVBD_2466c, Rv2466c
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 40.649Å b: 46.814Å c: 104.308Å
α: 90° β: 97.97° γ: 90°
R-values:
R R work R free
0.134 0.131 0.176
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Identification and validation of novel drug targets in Mycobacterium tuberculosis.
Singh et al. (2017)
3 more

3 mentions without citation

The Redox State Regulates the Conformation of Rv2466c to Activate the Antitubercular Prodrug TP053.
Albesa-Jové et al. (2015)
2 more