› 4zin

X-ray diffraction
1.67Å resolution

Genetically encoded Phenyl Azide Photochemistry Drive Positive and Negative Functional Modulation of a Red Fluorescent Protein

Released:
DOI: 10.2210/pdb4zin/pdb
PDB entry 4zin coloured by chain, front view.
PDB entry 4zin coloured by chain, side view.
PDB entry 4zin coloured by chain, top view.
Source organism: Anaplasma marginale
Primary publication:
Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein
Reddington SC, Driezis S, Hartley AM, Watson PD, Rizkallah PJ, Jones DD
RSC Adv 5 77734-77738 (2015)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MCherry fluorescent protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 217 amino acids
Theoretical weight: 24.78 KDa
Source organism: Anaplasma marginale
Expression system: Escherichia coli
UniProt:
  • Canonical: X5DSL3 (Residues: 11-229; Coverage: 93%)
Gene name: mCherry
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand EDO 1 x EDO
1 modified residue:
Ligand CH6 3 x CH6

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 43.28Å b: 103.43Å c: 150.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli

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