› 4zk9

X-ray diffraction
2.6Å resolution

The chemokine binding protein of orf virus complexed with CCL2

Released:
DOI: 10.2210/pdb4zk9/pdb
PDB entry 4zk9 coloured by chain, front view.
PDB entry 4zk9 coloured by chain, side view.
PDB entry 4zk9 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Orf Virus Chemokine Binding Protein in Complex with Host Chemokines Reveal Clues to Broad Binding Specificity.
Couñago RM, Knapp KM, Nakatani Y, Fleming SB, Corbett M, Wise LM, Mercer AA, Krause KL
Structure 23 1199-213 (2015)
PMID: 26095031

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146613 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Chemokine binding protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 30.37 KDa
Source organism: Orf virus (strain NZ2)
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2F862 (Residues: 17-286; Coverage: 94%)
Sequence domains: 35kD major secreted virus protein
C-C motif chemokine 2 Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P13500 (Residues: 24-99; Coverage: 100%)
Gene names: CCL2, MCP1, SCYA2
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P43212
Unit cell:
a: 78.802Å b: 78.802Å c: 185.613Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.238 0.281
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Targeting Chemokine-Glycosaminoglycan Interactions to Inhibit Inflammation.
Crijns et al. (2020)
6 more

8 mentions without citation

The binding and specificity of chemokine binding proteins, through the lens of experiment and computation.
Stark et al. (2022)
7 more