› 4zke

X-ray diffraction
2.25Å resolution

Crystal structure of the S. cerevisiae Ski7 GTPase-like domain, bound to GTP.

Released:
DOI: 10.2210/pdb4zke/pdb
PDB entry 4zke coloured by chain, front view.
PDB entry 4zke coloured by chain, side view.
PDB entry 4zke coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Saccharomyces cerevisiae Ski7 Is a GTP-Binding Protein Adopting the Characteristic Conformation of Active Translational GTPases.
Kowalinski E, Schuller A, Green R, Conti E
Structure 23 1336-43 (2015)
PMID: 26051716

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superkiller protein 7 Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 56.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08491 (Residues: 254-747; Coverage: 66%)
Gene names: SKI7, YOR076C, YOR29-27
Sequence domains: Elongation factor Tu GTP binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand GTP 1 x GTP
Ligand NA 1 x NA
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 91.15Å b: 123.106Å c: 104.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.239
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Ribosome as a Platform for mRNA and Nascent Polypeptide Quality Control.
Inada T. (2017)
6 more

3 mentions without citation

Targeting RNA for processing or destruction by the eukaryotic RNA exosome and its cofactors.
Zinder et al. (2017)
2 more