› 4zkj

X-ray diffraction
2.25Å resolution

Crystal structure of CRISPR-associated protein

Released:
DOI: 10.2210/pdb4zkj/pdb
PDB entry 4zkj coloured by chain, front view.
PDB entry 4zkj coloured by chain, side view.
PDB entry 4zkj coloured by chain, top view.
Source organism: Streptococcus pyogenes M1 476
Primary publication:
Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System.
Ka D, Lee H, Jung YD, Kim K, Seok C, Suh N, Bae E
Structure 24 70-79 (2016)
PMID: 26671707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 34.09 KDa
Source organism: Streptococcus pyogenes M1 476
Expression system: Escherichia coli
UniProt:
  • Canonical: J7M1H5 (Residues: 2-289; Coverage: 100%)
Gene names: M1GAS476_0831, cas1
Sequence domains: CRISPR associated protein Cas1

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P3121
Unit cell:
a: 95.205Å b: 95.205Å c: 210.843Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.211
Expression system: Escherichia coli

Quick links

Citations

1 review citation

CRISPR-Cas: Adapting to change.
Jackson et al. (2017)
11 other articles

1 mention without citation

The core Cas1 protein of CRISPR-Cas I-B in Leptospira shows metal-tunable nuclease activity.
Dixit et al. (2021)