› 4zkl

X-ray diffraction
2.34Å resolution

Crystal structure of human histidine triad nucleotide-binding protein 1 (hHINT1) complexed with JB419 (AP4A analog)

Released:
DOI: 10.2210/pdb4zkl/pdb
PDB entry 4zkl coloured by chain, front view.
PDB entry 4zkl coloured by chain, side view.
PDB entry 4zkl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic studies of the complex of human HINT1 protein with a non-hydrolyzable analog of Ap4A.
Dolot R, Kaczmarek R, Sęda A, Krakowiak A, Baraniak J, Nawrot B
Int J Biol Macromol 87 62-9 (2016)
PMID: 26905466

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 126 amino acids
Theoretical weight: 13.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49773 (Residues: 1-126; Coverage: 100%)
Gene names: HINT, HINT1, PKCI1, PRKCNH1
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

2 bound ligands:
Ligand AMP 2 x AMP
Ligand JB6 1 x JB6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 63.714Å b: 46.419Å c: 103.413Å
α: 90° β: 97.41° γ: 90°
R-values:
R R work R free
0.189 0.186 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The Many Faces of Histidine Triad Nucleotide Binding Protein 1 (HINT1).
Dillenburg et al. (2023)
2 other articles