› 4zlg

X-ray diffraction
1.75Å resolution

Cellobionic acid phosphorylase - gluconic acid complex

Released:
DOI: 10.2210/pdb4zlg/pdb
PDB entry 4zlg coloured by chain, front view.
PDB entry 4zlg coloured by chain, side view.
PDB entry 4zlg coloured by chain, top view.
Source organism: Saccharophagus degradans 2-40
Primary publication:
Crystal Structure and Substrate Recognition of Cellobionic Acid Phosphorylase, Which Plays a Key Role in Oxidative Cellulose Degradation by Microbes.
Nam YW, Nihira T, Arakawa T, Saito Y, Kitaoka M, Nakai H, Fushinobu S
J Biol Chem 290 18281-92 (2015)
PMID: 26041776

Function and Biology Details

Reaction catalysed: 
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate = alpha-D-glucose 1-phosphate + D-gluconate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative b-glycan phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 796 amino acids
Theoretical weight: 89.78 KDa
Source organism: Saccharophagus degradans 2-40
Expression system: Escherichia coli
UniProt:
  • Canonical: Q21MB1 (Residues: 1-788; Coverage: 100%)
Gene names: Sde_0906, cep94B
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand GCO 1 x GCO
Ligand GOL 2 x GOL
Ligand LGC 1 x LGC
Ligand SO4 2 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3121
Unit cell:
a: 106.993Å b: 106.993Å c: 186.544Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.201
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases.
Li et al. (2022)
11 other articles

1 mention without citation

Crystal Structure and Substrate Recognition of Cellobionic Acid Phosphorylase, Which Plays a Key Role in Oxidative Cellulose Degradation by Microbes.
Nam et al. (2015)