› 4zlh

X-ray diffraction
2Å resolution

Structure of the LapB cytoplasmic domain at 2 angstroms

Released:
DOI: 10.2210/pdb4zlh/pdb
PDB entry 4zlh coloured by chain, front view.
PDB entry 4zlh coloured by chain, side view.
PDB entry 4zlh coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
An Unexpected Duo: Rubredoxin Binds Nine TPR Motifs to Form LapB, an Essential Regulator of Lipopolysaccharide Synthesis.
Prince C, Jia Z
Structure 23 1500-1506 (2015)
PMID: 26190574

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142015 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipopolysaccharide assembly protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 38.87 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AB60 (Residues: 51-389; Coverage: 87%)
Gene names: ECs1853, Z2526, lapB, yciM
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 38.31Å b: 152.25Å c: 65.47Å
α: 90° β: 93.91° γ: 90°
R-values:
R R work R free
0.206 0.203 0.254
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects.
Klein et al. (2019)
3 more

5 mentions without citation

Regulation of the First Committed Step in Lipopolysaccharide Biosynthesis Catalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease.
Biernacka et al. (2020)
4 more