› 4zo1

X-ray diffraction
3.22Å resolution

Crystal Structure of the T3-bound TR-beta Ligand-binding Domain in complex with RXR-alpha

Released:
DOI: 10.2210/pdb4zo1/pdb
PDB entry 4zo1 coloured by chain, front view.
PDB entry 4zo1 coloured by chain, side view.
PDB entry 4zo1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism for signal transduction in RXR nuclear receptor heterodimers.
Kojetin DJ, Matta-Camacho E, Hughes TS, Srinivasan S, Nwachukwu JC, Cavett V, Nowak J, Chalmers MJ, Marciano DP, Kamenecka TM, Shulman AI, Rance M, Griffin PR, Bruning JB, Nettles KW
Nat Commun 6 8013 (2015)
PMID: 26289479

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145481 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thyroid hormone receptor beta Chain: X
Molecule details ›
Chain: X
Length: 252 amino acids
Theoretical weight: 28.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10828 (Residues: 210-461; Coverage: 55%)
Gene names: ERBA2, NR1A2, THR1, THRB
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chain: A
Molecule details ›
Chain: A
Length: 9 amino acids
Theoretical weight: 1.16 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 686-694; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2
Retinoic acid receptor RXR-alpha Chain: B
Molecule details ›
Chain: B
Length: 225 amino acids
Theoretical weight: 25.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19793 (Residues: 231-455; Coverage: 49%)
Gene names: NR2B1, RXRA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand T3 1 x T3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3121
Unit cell:
a: 63.252Å b: 63.252Å c: 225.806Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.236 0.281
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

The nuclear receptor superfamily: A structural perspective.
Weikum et al. (2018)
21 more

4 mentions without citation

Structural mechanism for signal transduction in RXR nuclear receptor heterodimers.
Kojetin et al. (2015)
3 more