› 4zow

X-ray diffraction
2.45Å resolution

Crystal structure of E. coli multidrug transporter MdfA in complex with chloramphenicol

Released:
DOI: 10.2210/pdb4zow/pdb
PDB entry 4zow coloured by chain, front view.
PDB entry 4zow coloured by chain, side view.
PDB entry 4zow coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Substrate-bound structure of the E. coli multidrug resistance transporter MdfA.
Heng J, Zhao Y, Liu M, Liu Y, Fan J, Wang X, Zhao Y, Zhang XC
Cell Res 25 1060-73 (2015)
PMID: 26238402

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug transporter MdfA Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 42.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AEY8 (Residues: 10-400; Coverage: 95%)
Gene names: JW0826, b0842, cmlA, cmr, mdfA
Sequence domains: Sugar (and other) transporter

Ligands and Environments

1 bound ligand:
Ligand CLM 1 x CLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 93.852Å b: 63.449Å c: 99.726Å
α: 90° β: 106.09° γ: 90°
R-values:
R R work R free
0.206 0.204 0.235
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

16 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
15 more

32 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
31 more