› 4zp0

X-ray diffraction
2Å resolution

Crystal structure of E. coli multidrug transporter MdfA in complex with deoxycholate

Released:
DOI: 10.2210/pdb4zp0/pdb
PDB entry 4zp0 coloured by chain, front view.
PDB entry 4zp0 coloured by chain, side view.
PDB entry 4zp0 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Substrate-bound structure of the E. coli multidrug resistance transporter MdfA.
Heng J, Zhao Y, Liu M, Liu Y, Fan J, Wang X, Zhao Y, Zhang XC
Cell Res 25 1060-73 (2015)
PMID: 26238402

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug transporter MdfA Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 42.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AEY8 (Residues: 9-400; Coverage: 96%)
Gene names: JW0826, b0842, cmlA, cmr, mdfA
Sequence domains: Sugar (and other) transporter

Ligands and Environments

2 bound ligands:
Ligand DXC 1 x DXC
Ligand LDA 2 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 94.652Å b: 64.235Å c: 101.35Å
α: 90° β: 110.02° γ: 90°
R-values:
R R work R free
0.211 0.21 0.23
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

20 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
19 more

28 mentions without citation

The Evolutionary Conservation of Escherichia coli Drug Efflux Pumps Supports Physiological Functions.
Teelucksingh et al. (2020)
27 more