› 4zpk

X-ray diffraction
3.6Å resolution

Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex with HRE DNA

Released:
DOI: 10.2210/pdb4zpk/pdb
PDB entry 4zpk coloured by chain, front view.
PDB entry 4zpk coloured by chain, side view.
PDB entry 4zpk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural integration in hypoxia-inducible factors.
Wu D, Potluri N, Lu J, Kim Y, Rastinejad F
Nature 524 303-8 (2015)
PMID: 26245371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118376 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Aryl hydrocarbon receptor nuclear translocator Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 43.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P53762 (Residues: 81-464; Coverage: 49%)
Gene name: Arnt
Sequence domains:
Endothelial PAS domain-containing protein 1 Chain: B
Molecule details ›
Chain: B
Length: 360 amino acids
Theoretical weight: 41.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P97481 (Residues: 3-361; Coverage: 41%)
Gene names: Epas1, Hif2a
Sequence domains:
DNA (5'-D(*GP*GP*CP*TP*GP*CP*GP*TP*AP*CP*GP*TP*GP*CP*GP*GP*GP*TP*CP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.53 KDa
DNA (5'-D(*CP*AP*CP*GP*AP*CP*CP*CP*GP*CP*AP*CP*GP*TP*AP*CP*GP*CP*AP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.36 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43
Unit cell:
a: 65.746Å b: 65.746Å c: 245.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.234 0.321
Expression system: Escherichia coli BL21

Quick links

Citations

44 review citations

Transcriptional architecture of the mammalian circadian clock.
Takahashi JS. (2017)
43 more

15 mentions without citation

Targeting Protein-Protein Interactions in the HIF System.
Wilkins et al. (2016)
14 more