› 4zq9

X-ray diffraction
2.6Å resolution

X-ray structure of AAV-2 OBD bound to AAVS1 site 3:1

Released:
DOI: 10.2210/pdb4zq9/pdb
PDB entry 4zq9 coloured by chain, front view.
PDB entry 4zq9 coloured by chain, side view.
PDB entry 4zq9 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into the Assembly of the Adeno-associated Virus Type 2 Rep68 Protein on the Integration Site AAVS1.
Musayev FN, Zarate-Perez F, Bishop C, Burgner JW, Escalante CR
J Biol Chem 290 27487-99 (2015)
PMID: 26370092

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118378 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein Rep68 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 211 amino acids
Theoretical weight: 24.41 KDa
Source organism: Adeno-associated virus 2 Srivastava/1982
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P03132 (Residues: 1-208; Coverage: 39%)
Gene name: Rep68
Sequence domains: Rep protein catalytic domain like
Structure domains: Replication Protein E1; Chain: A,
DNA (5'-D(*GP*CP*GP*CP*TP*CP*GP*CP*TP*CP*GP*CP*TP*CP*GP*CP*TP*GP*GP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*CP*CP*CP*AP*GP*CP*GP*AP*GP*CP*GP*AP*GP*CP*GP*AP*GP*CP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.48 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 71.097Å b: 137.255Å c: 79.249Å
α: 90° β: 111.72° γ: 90°
R-values:
R R work R free
0.206 0.205 0.23
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

4 review citations

Current Landscape of Heart Failure Gene Therapy.
Kieserman et al. (2019)
3 more

2 mentions without citation

Structural Insights into the Assembly of the Adeno-associated Virus Type 2 Rep68 Protein on the Integration Site AAVS1.
Musayev et al. (2015)
1 more